BLASTOCYST "PUMPING" IS A DETRIMENTAL FEATURE PREDICTING IMPLANTATION FAILURE: HIGHLY ACCURATE ASSESSMENT BY COMPUTER VISION ANALYSIS OF TIME-LAPSE VIDEOS

To determine for the first time, whether "pumping" or "weak contraction," defined as blastocoelic contraction by more than 8 microns in diameter, is associated with poor implantation rates, independent of other morphokinetic features. We analyzed 148,441 images from time-lapse sequences 317 expanded blastocysts, chosen transfer, known data. Using a computer vision algorithm, we were able to measure blastocyst approximated best-fit circle, steps microns. "pumping event" contraction" when diameter was smaller compared its image taken 40 minutes earlier. further "major pumping event," greater 16 microns, vs “minor event” where it but no also an "early occurred up 140 “late over The extent, number events, and stage blastulation at which measured embryos’ outcomes. Of 188 (59.3%) successfully implanted. blastocysts that implanted, early noted 27 (14%) total 73 average ± SD 110.7±4.2 hours. Early events 22 (17%) 129 failed implant overall 80 112.0±4.8 Major 25 times 14 (7%) implanted 110.5±7.7 hours vs. 38 18 implant, 111.7±4.6 significantly correlated failure (OR 1.87, P=0.03, least one major event, OR 2.42, P=0.04 two events). Multiple 2.07, P=0.02, three 2.55, four Weak contractions can be accurately using algorithms. These algorithms allowed us changes unprecedented accuracy "early" "major" found uncommon, they occur, are strongly predictive failure. Therefore, should preferably deselected if exhibit this behavior.